PMID: 6165241Jan 1, 1980Paper

Determination of glycosylated hemoglobins in neonatal blood by isoelectric focusing

American Journal of Hematology
M D FitzGerald, M N Cauchi

Abstract

Isoelectric focusing (IEF) with suitable "spacers" can be used to distinguish glycosylated hemoglobins from HbA and HbF in adults and infants, respectively. Using this technique we have shown that there is a good correlation between glycosylated hemoglobins measured by IEF and column chromatography (r = 0.778, P less than 0.001). There was no significant difference in the rate of glycosylation between adult and cord blood hemolysates following incubation with glucose in vitro: In both instances there was an increase from the normal levels of about 5% to 26% through 31% after 18 hours of incubation. The levels of glycosylated hemoglobins in infants of varying gestation, neonates, and normal adults was remarkably constant, varying from 4.7 +/- 1.1% to 6.1 +/- 1.6%. These results show that IEF can be useful in the quantitation of glycosylated hemoglobins, particularly in the presence of hemoglobins (eg, HbF) where standard chromatographic techniques cannot be used. IEF has the added advantage of using small quantities of blood, and allows a number of samples to be tested at the same time, thus facilitating the identification of abnormal hemoglobins.

References

Dec 1, 1976·FEBS Letters·R Flückiger, K H Winterhalter
Jul 1, 1979·The Journal of Clinical Investigation·K M SpicerM G Buse
Mar 27, 1979·Biochimica Et Biophysica Acta·E C AbrahamT H Huisman
Apr 16, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·V SaibeneG Pozza
May 20, 1978·The Medical Journal of Australia·R E DavisJ S Calder
Jan 1, 1978·American Journal of Hematology·L BeccariaP G Righetti
Jun 1, 1976·The Journal of Clinical Investigation·H F BunnP M Gallop
Aug 1, 1966·Biochemistry·W R Holmquist, W A Schroeder

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Citations

Mar 1, 1985·Diabetic Medicine : a Journal of the British Diabetic Association·W G JohnA E Jones
Jul 4, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Andrew S DavisonNorman B Roberts
Jun 1, 1984·Scandinavian Journal of Clinical and Laboratory Investigation·H A OlesenL Mølsted-Pedersen
Jan 1, 1984·Annals of Clinical Biochemistry·K Miedema, T Casparie

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