Determination of glyphosate and aminomethylphosphonic acid by sequential-injection reversed-phase chromatography: method improvements and application in adsorption studies

Analytical and Bioanalytical Chemistry
Erico A Oliveira PereiraJorge C Masini

Abstract

This paper describes a low-cost reversed-phase sequential injection chromatography method for the determination of glyphosate and aminomethylphosphonic acid in environmental samples. The method is based on the pre-column conversion of glyphosate to glycine by hypochlorite, followed by reaction with o-phthaldialdehyde in presence of 2-mercaptoethanol in borate buffer (pH 9.5) to produce the fluorescent 1-(2'-hydroxyethylthio)-2-N-alkylisoindole. In addition to producing detectable fluorescent indoles, the pre-column derivatization also decreases the polarity of the analytes, favoring their retention on a C18 monolithic column. The isocratic reversed-phase chromatography enabled the separation of both glyphosate and aminomethylphosphonic acid derivatives from polar compounds such as organic acids, humic substances, and carbohydrates which are commonly found in waters and soil extracts. This separation minimizes the laborious sample preparation procedures prior to the analysis. The linear response was observed for concentrations between 0.10 and 12.8 μM. The limits of detection and quantification were 0.03 and 0.10 μM (glyphosate), and 0.015 and 0.050 μM (aminomethylphosphonic acid). At the 0.10 μM concentration level, the relativ...Continue Reading

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Citations

Aug 8, 2020·Water Environment Research : a Research Publication of the Water Environment Federation·Chao LiJinkai Xue
Jul 26, 2019·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Erico A Oliveira PereiraJorge C Masini
Nov 19, 2019·Analytica Chimica Acta·Elias A G Zagatto, Fábio R P Rocha

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