Determination of gossypol enantiomer ratio in cotton plants by chiral higher-performance liquid chromatography

Journal of Agricultural and Food Chemistry
Quezia Bezerra CassAngela C De Pietro

Abstract

A celulose tris(3,5-dimethylphenylcarbamate) coated onto APS silica (Nucleosil, particle size, 7 microm; pore size, 500 A) was used under a reversed-phase condition to measure the enantiomeric ratios of gossypol enantiomers in cottonseeds, flowers, and roots in a number of cultivars samples of different Gossipium species. While unidimensional chromatography was used for measuring the enantiomeric ratio of all the samples of G. hirsutum, G. mustelinum, and in the seeds of G. barbadense, multidimensional chromatography was necessary for the analysis of samples of roots and flowers of G. barbadense. In the latter case, an ODS Hypersil column was used in the first dimension for sample clean up, and the enantiomers were resolved on the second dimension by the chiral column. As expected, all the seed samples of G. hirsutum and G. mustelinum showed the (P)-(+)-enantiomer in excess, whereas the seeds of G. barbadense showed the (M)-(-)-enantiomer. However, (P)-(+)-gossypol was found in enantiomeric excess in three samples examined of roots and in one of flower of G. barbadense. These results are discussed in this paper.

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Citations

Apr 11, 2012·Journal of Pharmaceutical and Biomedical Analysis·Kátia Roberta A BelazRegina V Oliveira
Aug 29, 2013·Biomedical Chromatography : BMC·María Eugenia León-GonzálezVanesa Guillén-Casla
Feb 24, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Andrea N L BatistaQuezia B Cass
Jun 3, 2005·Phytochemical Analysis : PCA
Dec 25, 2007·The Korean journal of laboratory medicine·Ki Hong KimDong Cheul Moon
Jul 28, 2005·Natural Product Reports·Braulio M Fraga
May 27, 2017·Current Medicinal Chemistry·Yun ZengDaocheng Wu
Jan 25, 2016·Science China. Life Sciences·Xiu TianChangqing Yang
Nov 20, 2016·Applied Microbiology and Biotechnology·Isabelle EffenbergerAndreas Schaller
Mar 15, 2008·Journal of Agricultural and Food Chemistry·Vanesa Guillén-CaslaLuis María Polo-Díez

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