Jun 30, 1975

Determination of GSH-DH activity (E.C.1.8.5.1) presence of the enzyme in different wheat varieties (author's transl)

Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung
W D KahntW Grosch

Abstract

2,6-dichlorophenol-indophenol is more stable in 75% ethanol than in aqueous solution and ascorbic acid reacts considerably faster in this media than glutathione. The activity determination of glutathione: dehydroascorbic acid oxidoreductase was based on these observations by a photometric measurement of the ascorbic acid resulting from the catalysis. Extracts from various types of wheat were tested and relatively high enzyme activities were found.

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Mentioned in this Paper

Photometry
Magnorbin
Catalysis
Glutathione Reductase
Hydrogen-Ion Concentration
Reduced Glutathione

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