PMID: 30143491Aug 26, 2018Paper

Determination of Hepatoma-Associated DL-Amino Acids Enantiomers by RP-HPLC with Fluorescence Detector: Application in Patients with Hepatocellular Carcinoma

Annals of Clinical and Laboratory Science
Huidan SunRong Luo

Abstract

Amino acids are increasingly being recognized as important signaling molecules in the pathogenesis of many diseases. We aim to establish a reversed-phase high-performance liquid chromatography with fluorescence detector (RP-HPLC-FLD) method for determination and quantification of hepatoma-associated DL-amino acids and to explore the relationship between amino acid concentrations and hepatocellular carcinoma (HCC). In this work, O-phthaldialdehyde (OPA) and N-isobutyryl-L-cysteine (NAC) served as the pre-column derivatization reagents which significantly shortened the detection time and improved the detection sensitivity. Chromatographic determination was achieved using a programmed gradient elution with a flow rate of 1.0 mL/min. The eluted solution was monitored by a fluorescence detector with an excitation wavelength at 350 nm and an emission wavelength at 450 nm. Under the optimum conditions, an excellent quantification of DL-threonine, alanine, tyrosine, valine, methionine and phenylalanine was achieved. Total analysis time was shortened to less than 25 min for one plasma sample and the linearity, recovery, intra- and inter-day precision were all meet the detection requirements of the DL-amino acids enantiomers in human pla...Continue Reading

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.