PMID: 9174269Apr 11, 1997Paper

Determination of hexahydrophthalic acid and methylhexahydrophthalic acid in plasma after derivatisation with pentafluorobenzyl bromide using gas chromatography and mass spectrometric detection

Journal of Chromatography. B, Biomedical Sciences and Applications
C H Lindh, B A Jönsson

Abstract

A method for the simultaneous determination of hexahydrophthalic acid (HHP acid) and methylhexahydrophthalic acid (MHHP acid) in human plasma was developed. The procedure was a rapid, single step extractive derivatisation with pentafluorobenzyl bromide as the derivatisation agent. The formed pentafluorobenzyl esters were analysed by gas chromatography-mass spectrometry in negative ion chemical ionisation mode with ammonia as the moderating gas. Deuterium-labeled HHP acid and MHHP acid were used as internal standards. The detection limit was 0.4 ng/ml for HHP acid (m/z 153) and 0.3 ng/ml for MHHP acid (m/z 365). The within-day precision of the method was between 2 and 3% and the between-day precision was between 3 and 12%. The overall recovery was between 65 and 83%. A comparison between HHP acid determinations with a previous and this method showed that the methods gave similar results. The method was applicable for analysis of plasma from occupationally exposed workers.

Citations

Feb 24, 2005·International Archives of Occupational and Environmental Health·Anna BlomqvistHans Welinder
Aug 1, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·C H Lindh, B A Jönsson
Oct 12, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·M TörnqvistP Rydberg
Jul 25, 2008·Rapid Communications in Mass Spectrometry : RCM·Christian H LindhBo A G Jönsson
Oct 29, 2008·Journal of Separation Science·Karim Asadpour-Zeynali, Naser Jalili-Jahani
Oct 4, 2008·Chemical Research in Toxicology·Marina C JeppssonChristian H Lindh
Apr 16, 2002·Chemical Research in Toxicology·Monica H KristianssonChristian H Lindh
May 11, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shigemi UedaIkuya Shibata

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