PMID: 9539972Apr 16, 1998Paper

Determination of HPRT mutant frequency and molecular analysis of T-lymphocyte mutants derived from coke-oven workers

Mutation Research
N ZanesiL Celotti

Abstract

We measured the frequency of mutant (MF) lymphocytes at the hprt locus in a population of 43 coke-oven workers exposed to PAH and in a group of 26 non-exposed workers. A non-significant increase in MF in the exposed group (19.0 +/- 16.3) compared to the non-exposed group (15.8 +/- 14.6) was observed. Moreover, when we considered smoking habits for the overall population, the MF values were higher, although not significantly, in smokers than in non-smokers. For some T-cell mutant clone structural alterations, splicing and coding errors were detected by PCR-based methods. We analysed 161 HPRT- clones, derived from exposed and non-exposed workers by multiplex-PCR and 56 HPRT- clones by reverse transcriptase-PCR. Overall, the percentages of the different types of gene alterations were similar in exposed and non-exposed subjects. Only the frequency of splice mutations in mutant clones derived from coke-oven workers was higher (22%) than in non-exposed donors (11%).

References

Jan 1, 1992·Environmental and Molecular Mutagenesis·B AnderssonB Lambert
Jan 1, 1990·Annual Review of Genetics·R J AlbertiniS H Robison
Apr 1, 1990·Genomics·A EdwardsW Ansorge
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R J AlbertiniW R Borcherding
Mar 1, 1995·Carcinogenesis·E ClonferoV Foà
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Jan 1, 1994·Environmental and Molecular Mutagenesis·J C FuscoeM M Moore
Dec 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·N ZanesiL Celotti

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