Determination of human immunoglobulin M rheumatoid factor by a solid-phase radioimmunoassay which uses human immunoglobulin G in antigen-antibody complexes.

Journal of Clinical Microbiology
B R ZiolaJ L Kalliomäki

Abstract

A solid-phase radioimmunoassay for the rapid determination of human immunoglobulin M (IgM) rheumatoid factor (RF) has been developed. Preparation of the solid phase for the assay involved the formation of complexes between respiratory syncytial virus-specific human IgG antibodies and virus antigen on the surface of polystyrene balls. Binding of serum RF to IgG in the immune complex was subsequently detected by 125I-labeled mu-chain-specific antibodies to human IgM. The amount of radioactive indicator antibody bound was converted to units of RF by comparison to the standard curve for an RF reference-serum pool. This assay should prove useful in studies of the physiological role of RF, since it can effectively measure low levels of circulating RF.

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Citations

Jul 6, 1984·Journal of Immunological Methods·I Teitsson, H Valdimarsson
Mar 26, 1982·Journal of Immunological Methods·B ZiolaK Penttinen
Jan 1, 1979·Journal of Immunological Methods·M GripenbergE Linder
Apr 1, 1981·Journal of Virological Methods·V A Sundqvist, B Wahren
Aug 1, 1982·Journal of Virological Methods·G A Lund, A A Salmi
Jul 30, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·R W DornerT L Moore
Jan 27, 1979·Lancet·M Vejtorp
Nov 1, 1982·Infection and Immunity·T ArnadottirA Salmi
Oct 1, 1986·Clinical Immunology and Immunopathology·A SalmiM Reunanen
Jan 1, 1983·Medical Microbiology and Immunology·H Tuokko, A Salmi
Feb 1, 1984·European Journal of Clinical Microbiology·H TuokkoA Salmi
Jan 1, 1981·Journal of Biological Standardization·R ZiegelmaierG Enders
Jun 1, 1988·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H Tuokko
Jun 1, 1980·Acta Pathologica Et Microbiologica Scandinavica. Section C, Immunology·B Ziola, H Tuokko

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