Determination of human transferrin concentrations in mouse models of neisserial infection

Journal of Immunological Methods
Yasser PereraRolando Pajon

Abstract

Transferrin constitutes the major protein involved in the transport of iron from the sites of absorption to the sites of storage and utilization. Despite the high affinity of transferrin for iron, most bacterial pathogens, such as the human restricted Neisseria meningitidis, have developed iron acquisition mechanisms. Several animal models of bacterial infection that include the exogenous supply of human transferrin have been implemented, and tests using transgenic mouse models are underway. Here we describe an ELISA sandwich procedure based on two monoclonal antibodies with negligible cross-reactivity to murine transferrin, to estimate human transferrin concentrations in mouse sera. The assay can detect as little as 10 ng/ml of human transferrin with coefficients of variation ranging from 1.6% to 4.4% (intra-assay) and 3.8% to 5% (inter-assay). The recovery values range from 90% to 110% in the assay working range (25-400 ng/ml). Human transferrin concentrations estimated in sera from 41 human transferrin transgenic mice ranged from 2 to 14 microg/ml. Further estimations of human transferrin levels in mouse sera of a previously described mouse model of N. meningitidis were also carried out. The intraperitoneal injection of 8 mg...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 1, 1992·Microbial Pathogenesis·F G MackinnonA Robinson
Jan 1, 1992·Critical Reviews in Microbiology·B R OttoD M MacLaren
Feb 1, 1990·Canadian Journal of Microbiology·A B Schryvers, G C Gonzalez
Nov 1, 1989·Molecular and Cellular Biology·R L IdzerdaR L Brinster
Jan 1, 1980·Annual Review of Biochemistry·P Aisen, I Listowsky
Sep 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·G S Baldwin
Apr 29, 1999·Developmental and Comparative Immunology·L Jaso-Friedmann, D L Evans
Mar 26, 2003·Infection and Immunity·Kyungcheol YiIgor Stojiljkovic
Aug 15, 2003·Molecular & Cellular Proteomics : MCP·Radhakrishna S TirumalaiTimothy D Veenstra
Mar 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Donna Perkins-BaldingIgor Stojiljkovic
May 25, 2004·Infection and Immunity·Geneviève Renauld-MongénieMarie-José Quentin-Millet
Dec 4, 2004·Journal of Neuroscience Research·John L BeardJames R Connor

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Citations

Sep 26, 2007·Infection and Immunity·Maria-Leticia ZarantonelliMuhamed-Kheir Taha
Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka

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