PMID: 1517607Aug 30, 1992Paper

Determination of IgE antibodies to the benzyl penicilloyl determinant. A comparison between poly-L-lysine and human serum albumin as carriers

Journal of Immunological Methods
M BlancaJ Garcia

Abstract

The influence of two different carriers, poly-L-lysine (PLL) and human serum albumin (HSA) in the binding of specific IgE antibodies to the benzyl penicilloyl hapten (BPO) was determined in a solid-phase assay. Serum samples from patients with a history of immediate reaction to penicillin and which had shown the presence of IgE antibodies to BPO were used. Benzyl penicilloyl derivatized cellulose discs were prepared using PLL of different molecular weight and HSA as carriers. These were treated with different molar ratios of benzyl penicillin. These reagents were compared for uptake of BPO-specific IgE using a pool of sera in a radioallergosorbent test (RAST) type assay. Two PLL systems and two HSA systems were finally compared using 26 individual sera. RAST values were compared by Kruskal-Wallis and Wilcoxon tests. The relationships between the four different assays were evaluated by determining Pearson correlation coefficients and the concordance by determining intraclass correlation coefficients (ICC). Analysis of means by the Wilcoxon test revealed significant differences (P less than 0.01) only when the different carrier assays were compared. The correlation coefficients between all the assays were significant (P less than...Continue Reading

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Citations

Mar 20, 2002·Current Allergy Reports·B A BaldoN H Pham
Sep 26, 2001·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·A RomanoM Blanca
Jul 30, 2002·Allergy·Antonino RomanoM Blanca
Jan 1, 1996·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M J TorresJ Garcia
Jan 20, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A RomanoM Blanca
Mar 12, 2011·Annales Françaises D'anesthèsie Et De Rèanimation·L GuillouxE Beaudouin
Apr 4, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M BlancaC Juárez
Feb 11, 2015·Nanomedicine : Nanotechnology, Biology, and Medicine·María I MontañezEzequiel Perez-Inestrosa
Sep 27, 2003·Allergy·M J TorresUNKNOWN EAACI Interest Group on Drug Hypersensitivity
Jan 26, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M BlancaA DeWeck
Dec 24, 2005·Expert Opinion on Drug Safety·Rebeca Rodriguez-PenaMaria Jose Torres
Aug 16, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Maria I MontañezEzequiel Perez-Inestrosa
Jul 2, 2003·Journal of Molecular Recognition : JMR·Francisco Sánchez-SanchoMiguel Blanca
Sep 25, 2002·Allergy·M J TorresM Blanca
Oct 11, 2016·Molecular and Cellular Probes·Samine RahmatpourEsmaeil Sadroddiny
Jul 6, 2017·International Journal of Molecular Sciences·Ozge SoyerBulent Enis Sekerel
Jun 12, 2018·Current Opinion in Allergy and Clinical Immunology·Miguel Blanca, Paul Whitaker
Sep 6, 2012·Allergy, Asthma & Immunology Research·Enrique GómezMiguel Blanca
Jan 1, 1996·European Journal of Immunology·E PadovanH U Weltzien
Apr 2, 2010·Allergy, Asthma & Immunology Research·Bernard Yu-Hor Thong
Jun 5, 2019·Expert Review of Clinical Immunology·Natalia Blanca-LopezMiguel Blanca
Jun 4, 2016·Current Opinion in Allergy and Clinical Immunology·Cristobalina MayorgaMaria I Montañez
Jun 26, 2015·Current Opinion in Allergy and Clinical Immunology·Miguel Blanca, Bernard Y-H Thong
Jun 22, 2016·Current Opinion in Allergy and Clinical Immunology·Miguel Blanca, Bernard Y-H Thong
Jun 28, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yolanda VidaEzequiel Perez-Inestrosa
Apr 27, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Noemi MolinaEzequiel Perez-Inestrosa
Nov 7, 2019·Current Pharmaceutical Design·Cristobalina MayorgaJ A Cornejo-García
Mar 16, 2021·Frontiers in Pharmacology·Cristobalina MayorgaEzequiel Perez-Inestrosa

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