Determination of illicit drugs and metabolites in oral fluid by microextraction on packed sorbent coupled with LC-MS/MS

Analytical and Bioanalytical Chemistry
Camilla MontesanoD Compagnone

Abstract

Oral fluid (OF) has become a valuable biologic specimen for toxicological analysis, especially in driving under the influence of drugs (DUID) investigations, because of easy and non-invasive collection procedures. In OF testing, being the sample volume is limited, multi-analyte procedures are particularly advantageous since they save time and resources. In this work, a procedure for the simultaneous analysis of 20 illicit drugs, belonging to the classes of cocaine, amphetamines, natural and synthetic opioids and hallucinogens, is presented. The sample preparation is based on microextraction by packed sorbent (MEPS), a novel technique which is based on the miniaturization of solid-phase extraction (SPE). The presented method, which includes all the most diffused illicit drugs and their metabolites, has been fully validated according to the Scientific Working Group for Forensic Toxicology (SWGTOX) guidelines. LLOQs ranged from 0.5 to 30 ng mL(-1) (diacetylmorphine); the presented method allows the detection of all the selected drugs quite below the cutoff values recommended by Substance Abuse and Mental Health Services Administration (SAMHSA) for abuse identification.

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Citations

Mar 12, 2016·Bioanalysis·Elisabeth Leere ØiestadRitva Karinen
Apr 21, 2016·Bioanalysis·Camilla Montesano, Manuel Sergi
Sep 13, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·T RosadoE Gallardo
Oct 11, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mohammad Mahdi MoeinMohamed Abdel-Rehim
Dec 25, 2016·Analytical and Bioanalytical Chemistry·Tiago RosadoEugenia Gallardo
Mar 22, 2018·Bioanalysis·Marta Concheiro, Marilyn A Huestis
Dec 3, 2017·Journal of the American Society for Mass Spectrometry·Ludmyla S TavaresAndréa R Chaves
Dec 13, 2018·Bioanalysis·Shahram SeidiReza Alizadeh
Jul 16, 2019·Forensic Sciences Research·Inês TabarraEugenia Gallardo
May 6, 2020·Journal of Analytical Toxicology·Amvrosios OrfanidisNikolaos Raikos
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Samir M AhmadJosé M F Nogueira
Aug 10, 2021·Preparative Biochemistry & Biotechnology·Xiaoyue ShanBingsheng Yang

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