PMID: 9187391Apr 25, 1997Paper

Determination of imipenem in plasma by high-performance liquid chromatography for pharmacokinetic studies in patients

Journal of Chromatography. B, Biomedical Sciences and Applications
L Garcia-CapdevilaJ Bonal

Abstract

A rapid and simple HPLC method is described for the determination of imipenem in human plasma. After blood collection, plasma was separated by centrifugation and immediately stabilized with 3-morpholinopropanesulfonic acid (MOPS) and ethylene glycol solution (1:1). The sample preparation, before injection into HPLC, was ultrafiltration. The mobile phase was boric acid buffer. The imipenem was detected at 300 nm and cilastatin sodium, coadministered, did not interfere. Calibration curves in human plasma were linear from 0.1 to 100 microg/ml. The limit of detection was 0.030 microg/ml. Inter-day precision at 0.1 microg/ml, determined as the coefficient of variation, was 6.26%. Only 250 microl of plasma was required in our assay. Due to the limited stability of imipenem [G.B. Smith et al., J. Pharm. Sci., 79 (1990) 732], stability studies in plasma were done to establish appropriate storage conditions. The assay was applied to pharmacokinetic studies in patients.

References

Jan 1, 1990·Journal of Pharmaceutical and Biomedical Analysis·G CarlucciM Bologna

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Citations

Jan 21, 2009·The Journal of Antimicrobial Chemotherapy·Sutep Jaruratanasirikul, Teeratad Sudsai
Jun 28, 2006·Antimicrobial Agents and Chemotherapy·Eric GiannoniDenis Bugnon
Mar 30, 2004·Antimicrobial Agents and Chemotherapy·Toshimi KimuraKazuo Yago
Jun 28, 2003·American Journal of Veterinary Research·Corrie W BarkerM A McCrackin Stevenson
Oct 1, 2015·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Huei-Ru LinKai-Chih Chang
Sep 13, 2015·Biomedical Chromatography : BMC·Irena Baranowska, Joanna Płonka
Sep 18, 2007·Journal of Pharmaceutical and Biomedical Analysis·Kayo IkedaMasafumi Taniwaki
Jun 22, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kayo IkedaMasao Kobayashi
Jan 13, 2015·Transplant Infectious Disease : an Official Journal of the Transplantation Society·C WelschN Allou
Apr 7, 2016·Journal of Chromatographic Science·Christina A Sutherland, David P Nicolau
Nov 13, 2007·Chronobiology International·Audrey BoulameryBenard Bruguerolle
Jul 4, 2009·American Journal of Health-system Pharmacy : AJHP : Official Journal of the American Society of Health-System Pharmacists·Bertrand PourroyDiane Braguer
Oct 20, 2005·The Journal of Antimicrobial Chemotherapy·Sutep JaruratanasirikulSomchai Sriwiriyajan

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