Feb 4, 1976

Determination of indomethacin in serum and urine by electron-capture gas-liquid chromatography

Journal of Chromatography
I Helleberg

Abstract

A specific and sensitive method for the quantitative determination of indomethacin in serum and urine is described. The drug is extracted at pH 5.0 with 1,2-dichloroethane and a portion of the organic extract is concentrated and made to react with diazoethane in diethyl ether. The ethyl ester derivative is analyzed by electron-capture gas-liquid chromatography, quantitation being achieved by comparison of peak areas for samples and standards, which are prepared in serum or urine and treated in the same manner as the samples. The limit of sensitivity is 50 ng/ml and the relative standard derivation for repeat determinations on the same sample is about 3%.

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Mentioned in this Paper

Urine
Osmosin
Dicom Derivation
Ethylene dichloride
Anesthetic ether
Indomethacin
Chromatography, Gas-Liquid
Esters
Ethyl ether
Urine - SpecimenType

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