PMID: 784Nov 1, 1975

Determination of inorganic pyrophosphatase in rat odontoblast layer by a radiochemical method

Scandinavian Journal of Dental Research
G Granström, A Linde


The enzyme inorganic pyrophosphatase (PPiase, EC from the odontoblastic layer of rat incisors has been studied by means of a radiochemical micromethod. The enzyme was incubated with 32P-pyrophosphate in tris-HCl buffer at 37 degrees C. The reaction was linear with time for at least 45 min, and the pH optimum was found to be 8.8, independent of the amount of pyrophosphate present. Heating the enzyme at 56 degrees C inhibited the enzyme activity rapidly, Mg2+ ions activated the enzyme by 15% at an ion concentration of 4 mM, while higher concentrations were inhibitory. Ca2+ ions and PO43-ions inhibited the enzyme at all concentrations. F- ions did not affect the PPiase at concentrations below 8 mM, whereas higher concentrations had an inhibiting effect. Urea was found to inhibit the enzyme at concentrations above 1.5 M, while EDTA was a strong inhibitor at very low concentrations. The characteristics of PPiase agree well with the properties of the enzyme nonspecific alkaline phosphatase (EC studied earlier.


May 1, 1975·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A Linde, B C Magnusson
Dec 1, 1971·Analytical Biochemistry·P H Cartier, L Thuillier
Jan 1, 1970·Calcified Tissue Research·J H WöltgensO L Bijvoet
Jun 1, 1961·The American Journal of Physiology·H FLEISH, W F NEUMAN
Jan 1, 1960·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H J DULCE
Jan 1, 1923·The Biochemical Journal·R Robison

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