Determination of Interleukin 27-Producing CD4(+) and CD8(+) T Cells for The Differentiation Between Tuberculous and Malignant Pleural Effusions

Scientific Reports
Ya-Lan LiuHuan-Zhong Shi

Abstract

The numbers of IL-27(+) CD4(+) and IL-27(+) CD8(+) T cells have been found to be increased in tuberculous pleural effusion (TPE) as compared with malignant pleural effusion (MPE). The objective of the present study was to investigate whether pleural IL-27(+) CD4(+) and IL-27(+) CD8(+) T cells can distinguish patients with TPE from those with MPE. Paired specimen of pleural fluid and peripheral blood were collected from 35 patients with TPE and 46 MPE. The numbers of IL-27(+) CD4(+) and IL-27(+) CD8(+) T cells were simultaneously determined by flow cytometry. Receiver operating characteristic curve analysis was used to evaluate the capacity of IL-27(+) CD4(+) and IL-27(+) CD8(+) T cells to differentiate TPE from MPE. The sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), positive predictive value (PPV), and negative predictive value (NPV) of IL-27(+) CD4(+) T cells were 94.3%, 93.5%, 14.46, 0.06, 91.7%, and 95.6%, respectively. The sensitivity, specificity, PLR, NLR, PPV and NPV of IL-27(+) CD8(+) T cells were 80.0%, 93.5%, 12.27, 0.21, 90.3% and 86.0%, respectively. The number of IL-27(+) CD4(+) in pleural fluid is a helpful diagnostic biomarker for the diagnosis of TPE, which performs b...Continue Reading

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Citations

Nov 19, 2013·Seminars in Immunology·Mark S Sundrud, Catherine Trivigno
Mar 15, 2018·Respiration; International Review of Thoracic Diseases·Qing LiuZhen Wang
Jul 12, 2020·QJM : Monthly Journal of the Association of Physicians·Qian ZhangWei Wu
Jun 4, 2021·Frontiers in Immunology·Yugo MoritaGowrishankar Muthukrishnan

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Methods Mentioned

BETA
biopsy
flow cytometry
FCS
enzyme linked immunosorbent assay

Software Mentioned

SPSS
BD FCS Diva
FCS Epress

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