PMID: 2497740Apr 15, 1989Paper

Determination of intracellular nitrate

The Biochemical Journal
J M RomeroM G Guerrero

Abstract

A sensitive procedure has been developed for the determination of intracellular nitrate. The method includes: (i) preparation of cell lysates in 2 M-H3PO4 after separation of cells from the outer medium by rapid centrifugation through a layer of silicone oil, and (ii) subsequent nitrate analysis by ion-exchange h.p.l.c. with, as mobile phase, a solution containing 50 mM-H3PO4 and 2% (v/v) tetrahydrofuran, adjusted to pH 1.9 with NaOH. The determination of nitrate is subjected to interference by chloride and sulphate when present in the samples at high concentrations. Nitrite also interferes, but it is easily eliminated by treatment of the samples with sulphamic acid. The method has been successfully applied to the study of nitrate transport in the unicellular cyanobacterium Anacystis nidulans.

Citations

Dec 22, 2007·Biotechnology and Bioengineering·Esperanza Del RíoMiguel G Guerrero
Jun 7, 2005·Biotechnology and Bioengineering·Esperanza Del RíoMiguel G Guerrero
Aug 8, 2002·FEMS Microbiology Reviews·José M Siverio
Sep 1, 1993·Molecular & General Genetics : MGG·A QuesadaE Fernández

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