Determination of inulin in plasma and urine by reversed-phase high-performance liquid chromatography

Journal of Chromatography. B, Biomedical Applications
R Dall'AmicoF Zacchello

Abstract

We report a new HPLC procedure for measuring inulin in plasma and urine. Samples after dilution are boiled in mild acidic conditions and then analyzed on a C18 column. Solvent system A is 3.2 mM HCl, pH 2.5, and B is acetonitrile-3.2 mM HCl (60:40, v/v), pH 2.5. The separation is carried out in 8 min with a flow-rate of 1.0 ml/min and the absorbance monitored at 280 nm. The relationship between inulin and the recorded peak area is linear from 0.2 to 3.2 mg/ml with a correlation coefficient of 0.999 for plasma and 0.999 for urine. Within-run precision, measured at three inulin concentrations, ranged from 0.9 to 1.7% in plasma and from 0.8 to 1.2% in urine. Between-run precision varied in plasma from 2.7 to 3.2% and in urine from 3.0 to 3.3%. Analytical recovery ranged from 102 to 107% in plasma and from 101 to 105% in urine, respectively. The method is sensitive, selective and only 30-microliters samples are required. Therefore, it could be used to evaluate the glomerular filtration rate even in small babies and to perform studies in animals.

References

Oct 1, 1955·The Journal of Clinical Investigation·M WALSERJ ORLOFF

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Citations

Sep 3, 2011·PLoS Neglected Tropical Diseases·Julie Nguyen-PouplinBridget Wills
Apr 4, 2002·Journal of Pharmaceutical and Biomedical Analysis·Thomas D NolinReginald F Frye
Sep 19, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·R MarsilioR Dall'Amico

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