Jul 1, 1976

Determination of ionized calcium in serum that has been exposed to air

Clinical Chemistry
D R WybengaD C Cannon


We examined changes in ionized calcium concentration in serum after its exposure to air. Samples with total protein concentrations ranging from 50 to 90 g/liter were equilibrated with CO2 in nitrogen (5/95, by vol) or CO2 alone, to produce pH values of 7.0 to 8.0. Ionized calcium was then measured with an Orion flow-through electrode system. Curves relating pH and ionized calcium concentration had statistically identical slopes regardless of protein concentration. A factor was derived, based on pH change, for correcting values for ionized calcium in serum exposed to air, and its validity was confirmed by comparing corrected values for samples allowed to stand at ambient temperature (23 degrees C) without anaerobic precautions with values initially obtained on anaerobic aliquots of the same samples.

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Mentioned in this Paper

Ionized calcium
Calcium ion
Regression Analysis
Ionized Calcium Assay
Drug Stability
Specimen Type - Electrode
Protein Concentration, Test Strip Measurement
Hydrogen-Ion Concentration

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