Determination of isoprene in human breath by thermal desorption gas chromatography with ultraviolet detection

Journal of Chromatography. B, Biomedical Applications
A W JonesC Tagesson

Abstract

We describe a new, highly sensitive and specific method for the analysis of isoprene (2-methyl-1,3-butadiene) in human breath. A known volume of expired air (150 ml) was drawn through a solid sorbent material to capture trace organic substances, followed by thermal desorption at 200 degrees C and subsequent determination of isoprene by gas chromatography with diode-array ultraviolet detection. The calibration plot was linear (r = 0.99) over a wide range of breath isoprene concentrations (0-12 nmol/l), and levels down to 0.10 nmol/l were easily measurable. In sixteen healthy subjects (six men and ten women), all of whom were non-smokers, the mean, median and spread of breath isoprene concentrations were 3.73, 3.36 and 1.60-10.33 nmol/l, respectively. No statistically significant differences in the concentrations of breath isoprene were observed between the sexes. The mean (+/- S.D.) concentration of breath isoprene in nine consecutive tests with the same subject was 3.69 +/- 0.60 nmol/l, and the coefficient of variation was 16.3%. Much larger variations in exhaled isoprene were seen during the day and also between days when the same subject was tested repeatedly. The excretion patterns of isoprene in human breath can be investig...Continue Reading

References

Jun 1, 1975·Archives of Environmental Health·J P ConkleB E Welch
Dec 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·W A Maltese
Jan 1, 1989·Life Sciences·A Cailleux, P Allain
Apr 30, 1981·Biochemical and Biophysical Research Communications·D GelmontJ F Mead
Oct 15, 1981·Biochemical and Biophysical Research Communications·D GelmontJ F Mead
Aug 1, 1994·Free Radical Biology & Medicine·C M KneepkensC C Roy
Jun 1, 1993·Biochemical Medicine and Metabolic Biology·A CailleuxP Allain

❮ Previous
Next ❯

Citations

Jun 9, 2001·Chemico-biological Interactions·G A Csanády, J G Filser
Feb 1, 2012·Journal of Chromatographic Science·Agnieszka UlanowskaBoguslaw Buszewski
Oct 1, 1995·Journal of Clinical Pathology·A W JonesC Tagesson
Jun 13, 2001·American Journal of Respiratory and Critical Care Medicine·S A Kharitonov, P J Barnes
Jul 22, 2010·Mass Spectrometry Reviews·Patrik Spaněl, David Smith
Nov 4, 2015·Critical Reviews in Analytical Chemistry·Mingjun XuYong Liu
Aug 24, 1999·Rapid Communications in Mass Spectrometry : RCM·P SpanelD Smith
Sep 25, 2012·Journal of Separation Science·Agnieszka UlanowskaBogusław Buszewski
Sep 5, 2013·Journal of Breath Research·Shih-Fang Chen, Mary-Grace C Danao
Mar 29, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·S DaviesD Smith
Apr 30, 2011·Analytical Chemistry·Toni LaurilaClemens F Kaminski
Feb 15, 1997·Analytical Chemistry·C Grote, J Pawliszyn
Mar 23, 2019·The Journal of Physical Chemistry Letters·Jessica P PorterfieldMichael C McCarthy
Mar 1, 2007·Analytical Chemistry·Shin-Ichi OhiraKei Toda

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.