PMID: 11339277May 8, 2001Paper

Determination of isoprostaglandin F2alpha type III in human urine by gas chromatography-electronic impact mass spectrometry. Comparison with enzyme immunoassay

Journal of Chromatography. B, Biomedical Sciences and Applications
Janine BessardG Bessard

Abstract

F2-Isoprostanes are stable lipid peroxidation products of arachidonic acid, the quantification of which provides an index of oxidative stress in vivo. We describe a method for analysing isoprostaglandin F2alpha type III (15-F2t-IsoP) in biological fluids. The method involves solid-phase extraction on octadecyl endcapped and aminopropyl cartridges. After conversion to trimethylsilyl ester trimethylsilyl ether derivatives, isoprostaglandin F2alpha type III is analysed by mass spectrometry, operated in electronic impact selected ion monitoring mode. We have compared enzyme immunoassay (EIA; Cayman, Ann Arbor, MI, USA) to this method with 30 human urine aliquots following the same extraction procedure in order to determine the agreement between both methods. Isoprostaglandin F2alpha type III concentrations determined with gas chromatography-mass spectrometry (GC-MS) did not agree with those determined with EIA. Our results suggest that GC-MS and EIA do not measure the same compounds. As a consequence, comparison of clinical results using GC-MS and EIA should be avoided.

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Citations

Jan 13, 2006·BioFactors·Yasukazu YoshidaEtsuo Niki
May 22, 2003·Free Radical Biology & Medicine·Vincent TongFrank S Abbott
Mar 17, 2007·Journal of Perinatology : Official Journal of the California Perinatal Association·S D ReuterC J Fernandes
Jul 25, 2009·Antioxidants & Redox Signaling·Rao Muralikrishna Adibhatla, James Franklin Hatcher
Aug 3, 2001·Journal of Cardiovascular Pharmacology·J L CracowskiG Bessard
May 14, 2005·Critical Care Medicine·Martin MatejovicIvan Novak
Oct 6, 2001·American Journal of Respiratory and Critical Care Medicine·J L CracowskiC Pison
Jan 8, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Edzard Schwedhelm, Rainer H Böger
Jun 9, 2006·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·B K ZedlerA R Tricker
Nov 5, 2003·Journal of Mass Spectrometry : JMS·Cinzia SignoriniGianluca Giorgi
May 9, 2006·Free Radical Biology & Medicine·Jean-Luc CracowskiEdzard Schwedhelm
Mar 20, 2012·The Science of the Total Environment·Christian G Daughton
Jul 4, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yi XiaoMaurice R O'Gorman
Apr 10, 2017·Redox Biology·Thomas J van 't ErveRonald P Mason
Feb 3, 2005·Free Radical Biology & Medicine·Olivier OrmezzanoJean Philippe Baguet
Dec 7, 2007·Molecular Aspects of Medicine·Mario ComportiConcetta Gardi
Apr 26, 2007·Journal of Lipid Research·Weiying YanMichael W Ogden
Mar 13, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Carl SofflerDiana M Hassel
Jul 27, 2001·Journal of Mass Spectrometry : JMS
Jan 26, 2002·The American Journal of Gastroenterology·Jean-Luc CracowskiJacques Fournet
Feb 8, 2003·Hypertension·Jean-Luc CracowskiJean-Michel Mallion
Sep 15, 2010·Free Radical Research·Corinne M SpickettNeven Zarkovic
Jul 7, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Andrew D WoolcockGeorge E Moore

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