Determination of kanamycin by electrophoretically mediated microanalysis with in-capillary derivatization and UV detection

Electrophoresis
Eliangiringa KaaleJos Hoogmartens

Abstract

An electrophoretically mediated microanalysis (EMMA) approach, used to perform on-line chemistry between two small molecules, has been characterized and optimized. The plug-plug type EMMA method involved electrophoretic mixing and subsequent reaction of nanoliter plugs of kanamycin-containing samples and 1,2-phthalic dicarboxaldehyde and mercaptoacetic acid within the confines of the capillary column, which acts as a microreactor. Analyses were performed by pressure-injecting a plug of kanamycin sandwiched in two reagent plugs. A potential of 375 Vcm(-1) was then applied to electrophoretically mix the two reactants, and an incubation time of up to 5 min allowed the reaction to proceed prior to the application of a separation potential of 588 Vcm(-1). UV detection was at 335 nm. The background electrolyte was 30 mM sodium tetraborate at pH 10.0, containing 16% of methanol. The method was validated in terms of linearity, limits of quantitation and detection, and precision. The method allows determination of kanamycin in bulk samples as a fully automated procedure.

Citations

Apr 7, 2004·Journal of Chromatography. a·Sona NovákováZdenek Glatz
Jan 2, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chang-Zhu YuWu-Er Gan
Mar 18, 2008·Journal of Chromatography. a·Yi-Fang LinSarah Y Chang
Sep 22, 2005·Rapid Communications in Mass Spectrometry : RCM·Eliangiringa KaaleAnn Van Schepdael
Dec 15, 2004·Electrophoresis·Feliciano Priego-Capote, Maria D Luque de Castro
Dec 1, 2006·Electrophoresis·Manuel Silva
Jan 20, 2006·Electrophoresis·Carmen García-Ruiz, María Luisa Marina
Nov 3, 2006·Electrophoresis·Juan Manuel Serrano, Manuel Silva
Mar 8, 2020·Journal of the Science of Food and Agriculture·Mustafa Oguzhan Caglayan
Sep 23, 2020·Chemical Communications : Chem Comm·Xiaowen LiuQingwang Xue

Related Concepts

1,2-phthalic dicarboxaldehyde
Kantrex
Microchemistry
Phthalic Acids
Spectrophotometry, Ultraviolet
Electrophoresis, Capillary

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