PMID: 9558136Apr 29, 1998Paper

Determination of lamotrigine in biologic materials by a simple and rapid liquid chromatographic method

Therapeutic Drug Monitoring
S RenW Zheng

Abstract

Lamotrigine (LTG), a newly introduced antiepileptic drug, appears to have potential therapeutic advantages for the treatment of patients with partial-onset seizures. Increasing clinical application and research of LTG demand a simpler and more rapid analytical procedure to determine LTG concentration in body fluids and tissues. The authors have developed an effective one-step procedure for sample preparation followed by high-performance liquid chromatography (HPLC) to quantitate LTG in plasma, urine, and brain tissues. Body fluids and brain homogenates were treated with cold acetonitrile to precipitate protein. The samples were fractionated on a 250 x 4.6 mm C18 reversed-phase column with an isocratic mobile system consisting of potassium phosphate buffer, acetonitrile, and methanol (70:16:14). The method had a LTG detection limit of 0.02 microg/ml in plasma and 0.03 microg/ml in urine. The coefficients of variation were <2.7% for intraday and 4.2% for interday analyses. The recovery of LTG added to plasma, urine, and brain homogenate ranged from 98% to 100%. The method was applied to a clinical study to determine plasma and urine concentrations of LTG in subjects receiving a single oral dose of LTG. The calculated pharmacokine...Continue Reading

References

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Citations

Apr 6, 2004·Environmental Health Perspectives·Mark G A OplerEzra S Susser
Apr 30, 2002·The Annals of Pharmacotherapy·Elaine Chong, L Lee Dupuis
May 22, 2002·Therapeutic Drug Monitoring·Manuela ContinAgostino Baruzzi
Dec 22, 1999·Therapeutic Drug Monitoring·M ContinA A Baruzzi
Jul 17, 2004·Therapeutic Drug Monitoring·Manuela ContinAgostino Baruzzi
Mar 7, 2021·Critical Reviews in Analytical Chemistry·Sajeeda SRuchi Verma
Mar 12, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Daniel F Chollet

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