Determination of linkage position and anomeric configuration in Hex-Fuc disaccharides using electrospray ionization tandem mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Jun XueK Matta

Abstract

Fixed-energy sequential tandem mass spectrometry (MS(n)) capabilities offered by quadrupole ion trap instruments have been explored in a systematic study of six isomers of Gal-Fucalpha-OBenzyl disaccharides. Under collision-induced dissociation (CID), sodiated molecular species generated in the positive-ion electrospray ionization mode yield simple and predictable mass spectra. Information on interglycosidic linkages and configurations can be deduced from the relative intensities of the selected diagnostic fragments arising from the glycosidic bond cleavages and corroborated by the fragments arising from cross-ring cleavages. As the CID patterns are not dependent on the number of prior tandem mass spectrometric steps, structures can be unambiguously assigned by matching the spectra with a library. The rules governing the fragmentation behavior of this class of oligosaccharides were tested for a representative isomeric disaccharide, Glcbeta1,3Fucalpha-OAllyl. The findings establish a basis for using MS(n) with a quadrupole ion trap instrument to elucidate structures of hexose-fucose subunits from more complicated oligosaccharides. Energy-resolved mass spectra were also acquired by CID tandem triple-quadrupole mass spectrometry. ...Continue Reading

Citations

Sep 4, 2007·Journal of the American Society for Mass Spectrometry·Shusaku DaikokuOsamu Kanie
Nov 9, 2004·Journal of Mass Spectrometry : JMS
Nov 1, 2005·Rapid Communications in Mass Spectrometry : RCM·Noritaka HashiiToru Kawanishi
Aug 5, 2006·Journal of Mass Spectrometry : JMS·K P Madhusudanan
Jun 15, 2007·Journal of Mass Spectrometry : JMS·Ahlam El FirdoussiJean-Yves Salpin
Nov 9, 2012·Rapid Communications in Mass Spectrometry : RCM·Elisabete V da CostaM Rosário M Domingues
Dec 14, 2017·Journal of the American Society for Mass Spectrometry·Hsu Chen HsuChi-Kung Ni
Oct 2, 2020·Rapid Communications in Mass Spectrometry : RCM·Yunpeng BaiChunbo Chen
Apr 24, 2021·Carbohydrate Research·Pia S Bruni, Stefan Schürch

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