Determination of lipoic acid in rat plasma by LC-MS/MS with electrospray ionization: assay development, validation and application to a pharamcokinetic study

Biomedical Chromatography : BMC
Ravi Kumar TrivediNuggehally R Srinivas

Abstract

A simple, sensitive and specific LC-MS/MS method for the determination of lipoic acid was developed and validated over the linearity range 5-1000 ng/mL (r2 > 0.99) with 200 microL rat plasma using rosigliatzone as an internal standard (IS). The assay procedure involved a simple one-step liquid-liquid extraction of lipoic acid and IS from plasma into ethyl acetate. The organic layer was separated and evaporated under a gentle stream of nitrogen at 40 degrees C. The residue was reconstituted in the mobile phase and injected onto a Hichrom RPB column (4.6 x 250 mm, 5 microm). Separation of lipoic acid and IS was achieved with a mobile phase consisting of 0.05 M formic acid:acetonitrile (40:60, v/v) at a flow rate of 1.0 mL/min. The API-3000 LC-MS/MS was operated under the multiple reaction monitoring mode (MRM) using the electrospray ionization technique. Positive and negative ion acquisition within the same chromatographic run was used in the present method. For lipoic acid a pseudo-molecular ion transition pair was acquired in negative polarity, whereas for IS the transition pair was acquired in positive polarity. Quantitation was determined for both analyte and IS in MRM scan mode. Absolute recovery of lipoic acid and IS was >7...Continue Reading

References

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Citations

Jun 18, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hideyuki YoshidaMasatoshi Yamaguchi
Dec 20, 2014·American Journal of Physical Anthropology·Brad A Chadwell, Jesse W Young
Aug 14, 2015·Bioanalysis·Kamila BorowczykGrażyna Chwatko
Jan 10, 2017·PloS One·Yirong SunHaihong Wang
Apr 19, 2017·Electrophoresis·Paweł Kubalczyk, Rafał Głowacki
Sep 21, 2007·Journal of Pharmaceutical and Biomedical Analysis·Arjumand I DurraniGerhard Sontag
Jul 22, 2005·Journal of Mass Spectrometry : JMS

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