Determination of lomerizine in human plasma by liquid chromatography/tandem mass spectrometry and its application to a pharmacokinetic study

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Yulong RenJianying Liang

Abstract

A rapid, sensitive and selective high performance liquid chromatography-electrospray ionization-tandem mass spectrometry method (HPLC-ESI-MS/MS) was developed and validated for the determination and pharmacokinetic investigation of lomerizine in human plasma. Protein precipitation process was used to extract lomerizine from human plasma. Plasma samples were separated by HPLC on an Agela Venusil XBP Phenyl column (100 mm × 2.1 mm, 5 μm) using a mobile phase consisting of methanol-2mM ammonium acetate-formic acid (70:30:0.1, v/v/v) and the flow rate was set at 0.35 mL/min. The total run time was 4.0 min and the elution of lomerizine was at 1.9 min. The detection was performed on a triple quadrupole tandem mass spectrometer in the multiple reaction-monitoring (MRM) mode using the respective [M+H](+) ions m/z 469.2→181.0 for lomerizine and m/z 405.2→202.9 for the I.S. The calibration curve was linear over the range of 0.1-25 ng/mL (r(2)>0.99) with a limit of quantitation (LOQ) of 0.1 ng/mL. The intra- and inter-day precision (relative standard deviation, RSD) values were below 9.65% and the mean accuracy was from 99.00 to 103.00% at four quality control levels. Lomerizine was stable during stability studies, i.e., long term, auto-s...Continue Reading

References

Jun 22, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hui-Min XiaJun Chen
Jul 24, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yu-Guan WenDe-Wei Shang
Sep 25, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ying-yi LiNing-sheng Wang
Nov 6, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ruina GaoXiaoyan Chen

Related Concepts

Lomerizine dihydrochloride
Calcium Channel Blockers
High Pressure Liquid Chromatography Procedure
Piperazines
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Methanol
High Pressure Liquid Chromatography Procedure
Ions
Drug Kinetics

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