Determination of lovastatin in human plasma by ultra-performance liquid chromatography/electrospray ionization tandem mass spectrometry

Biomedical Chromatography : BMC
Dong WangFamei Li

Abstract

A fast and sensitive ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination of lovastatin in human plasma. With simvastatin as internal standard, sample pretreatment involved one-step extraction with n-hexane-methylene dichloride-isopropanol (20:10:1, v/v/v) of 0.5 mL plasma. Chromatographic separation was carried out on an Acquity UPLC BEH C(18) column with mobile phase consisting of acetonitrile-water (containing 5 mmol/L ammonium acetate; 85:15, v/v) at a flow-rate of 0.35 mL/min. The detection was performed on a triple-quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) via electrospray ionization source with positive mode. The analysis time was shorter than 1.7 min per sample. The standard curve was linear (r2>or=0.99) over the concentration range 0.025-50.0 ng/mL with a lower limit of quantification of 0.025 ng/mL. The intra- and inter-day precision values were below 11% and the accuracy (relative error) was within 6.0% at three quality control levels. This is the first method of MS with MRM coupled to UPLC for the determination of lovastatin, which showed great advantages of high sensitivity, selectivity and high sample throughput. It...Continue Reading

References

Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A W AlbertsJ Springer
Jul 9, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mona I ChurchwellDaniel R Doerge
Aug 31, 2006·Rapid Communications in Mass Spectrometry : RCM·Madgalene KalovidourisIrene Panderi
Sep 12, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiaohong SunFamei Li
Apr 17, 2007·Journal of Pharmaceutical and Biomedical Analysis·Ramakrishna NirogiVishwottam Kandikere

❮ Previous
Next ❯

Citations

Feb 10, 2016·Bioanalysis·Hana VlčkováLucie Nováková
Jun 11, 2009·Photochemistry and Photobiology·Fernando LorenzoNorman S Allen
Sep 2, 2009·Biological & Pharmaceutical Bulletin·Mehrdad HamidiMohammad-Ali Shahbazi
May 11, 2010·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Mohie M K Sharaf El-DinMohamed M Y Kaddah

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anxiety Disorders

Discover the latest research on anxiety disorders including agoraphobia, panic disorder, obsessive-compulsive disorder, and post-traumatic stress disorder here.