Determination of lovastatin in human plasma by ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry and its application in a pharmacokinetic study

Journal of Pharmaceutical and Biomedical Analysis
Haiyan YuanHuande Li

Abstract

A selective, rapid and sensitive ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the quantitative determination of lovastatin in human plasma and its application in a pharmacokinetic study. With mycophenolate mofetil as internal standard, sample pretreatment involved a one-step extraction with tert-butyl methyl ether of 0.2 ml plasma. The analysis was carried out on an ACQUITY UPLCTM BEH C18 column (50 mm x 2.1 mm, i.d., 1.7 microm) with flow rate of 0.35 ml/min. The mobile phase was 20% water and 80% acetonitrile (v/v). The detection was performed on a triple-quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI). Linear calibration curves were obtained in the concentration range of 0.08-24.50 ng/ml, with a lower limit of quantification of 0.08 ng/ml. The intra- and inter-day precision (RSD) values were below 15% and accuracy (RE) was -7.6 to 9.3% at all QC levels. The method was applicable to clinical pharmacokinetic study of lovastatin in healthy volunteers following oral administration.

References

Jul 9, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mona I ChurchwellDaniel R Doerge

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Citations

Dec 25, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Vinicius Freire FagundesChristian Fernandes
Feb 10, 2016·Bioanalysis·Hana VlčkováLucie Nováková
Dec 10, 2014·Journal of Pharmaceutical and Biomedical Analysis·Marta Hryniewicka, Barbara Starczewska
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Konstantinos TsiantasPanagiotis Zoumpoulakis

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