PMID: 9188821May 9, 1997Paper

Determination of LSD and N-demethyl-LSD in urine by liquid chromatography coupled to electrospray ionization mass spectrometry

Journal of Chromatography. B, Biomedical Sciences and Applications
H HojaG Lachâtre

Abstract

A sensitive and highly specific method for the determination of LSD and N-demethyl-LSD in urine, using combined liquid chromatography and mass spectrometry (LC-MS) with electrospray ionization, has been developed. Extrelut-3 extraction cartridges were used for a basic sample clean-up. Elution was obtained by toluene-diethyl ether (60:40, v/v). A Nucleosil C18 (150 X 1 mm I.D.) reversed-phase column was used for the chromatographic separation, together with a mixture of 2 mM ammonium formate buffer (pH 3) and acetonitrile (70:30, v/v) as mobile phase. Recoveries were 93 and 80%, detection limits 0.025 and 0.035 ng/ml for LSD and N-demethyl-LSD, respectively. Intra-assay precision, studied at four concentrations, was better than 9% at the ng/ml range and better than 14% at 0.10 ng/ml for both compounds. Limits of quantitation were 0.05 and 0.10 ng/ml for LSD and N-demethyl-LSD, respectively. Reproducibility was good and linearity excellent for LSD in the range from 0.05 to 20 ng/ml (r>0.9999, n=7).

Citations

Feb 24, 2006·International Journal of Legal Medicine·Donata FavrettoSanto Davide Ferrara
Nov 26, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·P Marquet, G Lachâtre
Oct 25, 2011·Forensic Science International : Synergy·Jean-michel GaulierGérard Lachâtre
Sep 10, 2009·Journal of Separation Science·Florence Chapuis-HugonValerie Pichon
Sep 15, 2000·Mass Spectrometry Reviews·J F Van BocxlaerA P De Leenheer
Jul 4, 2019·Drug Metabolism Reviews·Rui Filipe Libânio Osório Marta
Jul 31, 2001·Therapeutic Drug Monitoring·K Bodin, J O Svensson
Nov 26, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·M J Bogusz
Nov 26, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·S A ReuschelR L Foltz

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