PMID: 11334351May 4, 2001Paper

Determination of lysophosphatidic acids by capillary electrophoresis with indirect ultraviolet detection

Journal of Chromatography. B, Biomedical Sciences and Applications
Y L Chen, Y Xu

Abstract

Lysophosphatidic acid (LPA) is the simplest form of lysophospholipid. Molecular species of LPA have been identified as the potent components in the ovarian cancer activation factor. The elevated plasma LPAs may be used as potential biomarkers for the early detection of ovarian cancer. This paper is the first report on the quantitative analysis of molecular species of LPA using capillary electrophoresis. In this work, the separation of LPAs was achieved within 14 min in an adenosine monophosphate-borate-methanol-water solution, and the measurement was accomplished by indirect UV detection. With LPA (D) as internal standard, the method had linear calibration ranges for LPAs from 2.8 to 75 microM. The detection limits for various molecular species of LPA were from 1.2 to 2.3 microM by the pressure injection at 3.45 kPa for 5 s. The method had been applied to serum fortified with LPA (S), LPA (O), LPA (P), and LPA (M) and the recoveries ranged from 83 to 112%.

References

Jun 1, 1993·Trends in Pharmacological Sciences·M E Durieux, K R Lynch
Jun 15, 1995·Analytical Chemistry·Y Xu
Apr 26, 1996·Journal of Chromatography. B, Biomedical Applications·H ChenM P Ip
Aug 26, 1998·Journal of Chromatographic Science·E HaddadianN D Danielson
Sep 5, 1998·JAMA : the Journal of the American Medical Association·Y XuG Casey
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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Citations

Mar 13, 2014·Analytical Biochemistry·Anna JesionowskaBarbara Dolegowska
Jun 28, 2011·Journal of Pharmaceutical and Biomedical Analysis·S P BathenaY Alnouti
Dec 18, 2014·Chemical Communications : Chem Comm·Lei WangRobert M Strongin
Aug 4, 2018·Electrophoresis·Véréna PoinsotFrançois Couderc
Mar 21, 2007·Electrophoresis·Fei GaoHuwei Liu
Dec 5, 2013·Electrophoresis·Cristina MontealegreMaria Fiorenza Caboni
Sep 27, 2013·The Analyst·Jialu WangRobert M Strongin
Feb 23, 2020·Biosensors·Brian De La Franier, Michael Thompson
Apr 13, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Niina AaltonenMarko Lehtonen

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