Determination of malachite green residues in rainbow trout muscle with liquid chromatography and liquid chromatography coupled with tandem mass spectrometry

Food Additives and Contaminants
Kirsi HalmeKimmo Peltonen

Abstract

A method for the determination of malachite green and its major metabolite leucomalachite green in rainbow trout muscle is reported with limits of detection of 0.8 and 0.6 microg kg(-1), respectively. Residues were extracted with an acetonitrile-acetate buffer mixture and partitioned into methylene chloride. Clean-up of the extracts was performed on alumina and propylsulfonic acid solid-phase extraction columns using the automated solid-phase extraction system. The chromatographic separation of malachite green and leucomalachite green was achieved on a Chromspher 5B column using an acetonitrile-acetate buffer mobile phase. Leucomalachite green was converted to malachite green by post-column oxidation before spectrophotometric detection at 600 nm. The mean recoveries of malachite green and leucomalachite green from control rainbow trout muscle spiked at 2-50 microg kg(-1) were 65% (range 63.4-65.9%, relative standard deviation 3.9-16.1%) and 74% (range 58.3-82.6%, relative standard deviation 3.3-11.4%), respectively. Qualitative confirmation of the determined residues was performed with liquid chromatography coupled with tandem mass spectrometry detection with limits of detection of 2.5 and 1 microg kg(-1) for malachite green an...Continue Reading

References

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Apr 23, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Aldert A Bergwerff, Peter Scherpenisse

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Citations

Jun 26, 2009·Analytical and Bioanalytical Chemistry·Jonas Augusto Rizzato PaschoalSusanne Rath
Dec 4, 2012·Environmental Monitoring and Assessment·R SahraeiH Noorizadeh
Oct 1, 2009·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·J A R PaschoalS Rath
Jun 15, 2010·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Jun Bae LeeMeehye Kim
Apr 17, 2008·Analytical and Bioanalytical Chemistry·T Le Goff, S Wood
Jan 25, 2006·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Passapol NgamukotSuchada Chuanuwatanakul
Sep 29, 2009·Journal of Chromatography. a·Brian KinsellaMartin Danaher
Aug 26, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kirsi HalmeKimmo Peltonen
Feb 15, 2014·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Kamran AbroShahnaz Perveen
May 15, 2018·Critical Reviews in Analytical Chemistry·Xinhui ZhouDaoliang Li
Jan 27, 2005·Journal of Mass Spectrometry : JMS

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Software Mentioned

MassLynx
735 Sampler
Empower

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