Determination of manassantin B in rat plasma using a high performance liquid chromatography with fluorescence detection and its quantitative application to pharmacokinetic study

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Jae-Young LeeHyun-Jong Cho

Abstract

A simple, sensitive, rapid, and reproducible analytical method of manassantin B in rat plasma by high performance liquid chromatography with fluorescence detection (HPLC-FL) was developed for its application to pharmacokinetic study in rats. Valsartan (VST) was used as an internal standard (IS) in this quantitative analytical method. Manassantin B and VST were extracted by simple and efficient protein precipitation method. Manassantin B was detected at 282/322nm (excitation/emission) wavelengths using FL detector. The chromatographic separation was obtained with reverse phase C18 column and the mobile phase composed of potassium phosphate buffer containing 0.025% trifluoroacetic acid (pH 2.5; 5mM) and acetonitrile including 0.025% trifluoroacetic acid (20:80, v/v) at 1.0mL/min flow rate. The linearity was established at 25.0-10000ng/mL and the lower limit of detection (LLOD) was 7ng/mL. The intra- and inter-day accuracy and precision values of manassantin B were within±15% of the theroretical values and <9% from the nominal concentrations, respectively. Accuracy and precision values of manassantin B after stability tests were also within the acceptable ranges. Developed assay was also successfully applied to pharmacokinetic stu...Continue Reading

References

Jun 22, 2005·Biochemical and Biophysical Research Communications·Chowdhury Faiz HossainYu-Dong Zhou
Mar 4, 2008·Biological & Pharmaceutical Bulletin·Chang-Seob SeoJong-Keun Son
Dec 17, 2009·Journal of Pharmaceutical and Biomedical Analysis·Hong-Jiang ChenBao-Chang Cai
Jul 24, 2012·Phytotherapy Research : PTR·Mi Jeong SungDae Young Kwon
Oct 12, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mohammed G KassemGamal A E Mostafa
Mar 15, 2015·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Ha Jin JeongSonghee Jeon

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