Determination of mangiferin in rat eyes and pharmacokinetic study in plasma after oral administration of mangiferin-hydroxypropyl-beta-cyclodextrin inclusion

Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics
Hong ZhangHao Cui

Abstract

To determine whether mangiferin can enter the eye, because the eye possesses the unique blood-ocular barrier. Due to the low solubility of mangiferin, hydroxypropyl-beta-cyclodextrin was applied to improve the absorbance. A reversed-phase high-performance liquid chromatography assay for mangiferin in rat plasma and eye fluid was developed. The mobile phase consisted of methanol-0.6% glacial acetic acid (27:73, v/v) at a flow rate of 1.0 mL/min. Peak areas of mangiferin were linear over a concentration range of 0.40-20.00 microg/mL in blood samples and a range of 0.10-5.00 microg/mL in eye samples, respectively. Intra- and interassay precision and accuracy of mangiferin fell well within the predefined limits of acceptability (<15%). The recovery was 81.12%-87.18% in the plasma and 79.96%-85.52% in the eye, respectively. The analytical method had excellent sensitivity using a small sample volume (20 microL) with the lower limit of quantification, 0.4 and 0.10 microg/mL for plasma and eye, respectively. Pharmacokinetic parameters of mangiferin in plasma were obtained as follows: T(max) = 7 h, C(max) = (4.43 +/- 0.62)microg/mL. The time point of 7 h was chosen for eye sample detection, as it had maximum plasma concentration. The co...Continue Reading

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Citations

May 20, 2020·Journal of Complementary & Integrative Medicine·Saurabh MittalSanjula Baboota
May 4, 2017·Lipids in Health and Disease·Muhammad ImranMuhammad Tauseef Sultan
Dec 22, 2020·Neurochemistry International·Vaibhav WaliaNeeraj Kumar Sethiya
Jan 19, 2021·Drug Development Research·Tingjun LiuAnkang Hu
Oct 5, 2021·Critical Reviews in Food Science and Nutrition·Suhuan MeiXiumin Chen

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