Determination of melanin in human hair by photoacoustic spectroscopy

Analytical Biochemistry
T WatanabeH Nakazawa

Abstract

A highly sensitive photoacoustic spectroscopy for the determination of melanin in human hair has been developed. Both synthetic melanin and human hair were treated with 50% sulfuric acid. The melanin suspension was passed through the membrane filter to collect melanin, and the filter was transferred into a homemade photoacoustic cell, followed by measurement of the photoacoustic signals produced by 10 mW He-Ne laser beam. The good correlation between the method described in this paper and absorption spectrophotometry was confirmed quantitatively. The linearity observed between the photoacoustic signal and the weight of samples was in the range of 0.1-20 micrograms for synthetic melanin and 10-800 micrograms for human hair, with detection limits of 5 ng and 0.38 microgram, respectively.

References

Aug 17, 1973·Science·A Rosencwaig

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Citations

Oct 23, 2016·Pigment Cell & Melanoma Research·Bruno FernandesArtur Cavaco-Paulo
Mar 4, 2014·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hiroyuki Nakazawa
Nov 8, 2018·The Analyst·Naricha PupinyoWanida Laiwattanapaisal
Jan 30, 2019·Scientific Reports·Soobin ChungJi Youn Lee
Feb 9, 2021·Global Challenges·Hanaa A GalebJohn G Hardy
Oct 11, 2003·Biotechnology Advances·V P GrishkoB R Glick

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