Determination of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine and alprazolam in human plasma by liquid chromatography-electrospray ionization mass spectrometry

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
O QuintelaM López-Rivadulla

Abstract

A fast liquid chromatographic assay with mass spectrometric detection (LC/MS) has been developed and validated for the simultaneous determination of methadone (MT), its primary metabolite, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) and alprazolam, in human plasma. The extraction procedure was performed with automatic solid phase extraction, and the compounds were separated with a Sunfire column using a gradient mode. Deuterated analogues for all of the analytes of interest were used for quantitation. Limits of detection (LOD) were established between 0.5 and 1 ng/ml. Linearity was obtained over a range of 2-2,000 ng/ml with an average correlation coefficient (R(2)) of >0.99. Intra- and inter-batch coefficients of variation and relative mean errors were less than 10% for all analytes and concentrations. The recoveries were higher than 50.0% in all cases. The method proved to be suitable for evaluation of plasma obtained from patients enrolled in a MT-maintenance program who are frequently treated with alprazolam as a sedative.

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Citations

Nov 26, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Eleni KalogriaIrene Panderi
Oct 19, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Laura MercoliniMaria Augusta Raggi
Mar 18, 2015·Journal of Pharmaceutical and Biomedical Analysis·Karolina PersonaWojciech Piekoszewski
Jan 3, 2007·Journal of Mass Spectrometry : JMS
Apr 21, 2019·Journal of Chromatographic Science·Zeynab TavakoliMir Mohammad Alavi Nikje
Jan 20, 2011·Clinical Chemistry·Ana de CastroMarilyn A Huestis
Mar 15, 2011·Biomedical Chromatography : BMC·Mitsuhiro Nakamura

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