Determination of methamphetamine and related compounds by capillary electrophoresis with UV and laser-induced fluorescence detection

Journal of Chromatography. a
N KurodaK Nakashima

Abstract

Methods for the simultaneous determination of methamphetamine (MP) and its related compounds (MPs) using capillary electrophoresis (CE) with UV absorbance and laser-induced fluorescence (LIF) detection are described. In UV detection, MPs were applied to CE without any derivatization procedure and detected at 210 nm for a rapid and simple analysis. Capillary zone electrophoresis (CZE) and electrokinetic capillary electrophoresis (MEKC) were used. MP, amphetamine (AP), 1-phenylethylamine (1-PA as an I.S.), 2-phenylethylamine (2-PA), 4-hydroxymethamphetamine (4-HMP) and 4-hydroxyamphetamine (4-HAP) were separated within 15 min by both CZE and MEKC. Detection limits of MPs were in the range 48-72 fmol/injection for CZE and 85-191 fmol/injection for MEKC. MEKC was successfully applied to the determination of MPs in urine. For a highly sensitive analysis, LIF detection was also examined using 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) as a fluorescent derivatization reagent. By the method, in which MPs derivatives were separated within 45 min by MEKC, 22-40 amol/injection of primary amines (AP, 4-HAP and 2-PA) and 690 amol/injection of MP and 300 amol/injection of 4-HMP were detected. The concentration of MP and AP in 50 microlite...Continue Reading

References

Jul 1, 1989·Journal of Analytical Toxicology·R A CoxG L Pshak
Oct 1, 1996·Journal of Analytical Toxicology·J FayV Spiehler

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Citations

Feb 24, 2001·Electrophoresis·J C WatervalW J Underberg
Aug 17, 2001·Biomedical Chromatography : BMC·S UchiyamaK Imai
Jan 14, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kenichiro NakashimaMitsuhiro Wada
May 24, 2006·Electrophoresis·Rukhsana JabeenJohn Petersen
May 8, 2007·Electrophoresis·Phillip HasemannHermann Wätzig
Mar 27, 2001·Biomedical Chromatography : BMC·C MarchP S Mukherjee
Mar 4, 2000·Journal of Pharmaceutical and Biomedical Analysis·N PizarroR de la Torre

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