Determination of methionine and selenomethionine in yeast by species-specific isotope dilution GC/MS

Analytical Chemistry
Lu YangRalph E Sturgeon

Abstract

A method for the simultaneous determination of methionine (Met) and selenomethionine (SeMet) in yeast using species-specific isotope dilution (ID) gas chromatography/mass spectrometry (GC/MS) is described. Samples were digested by refluxing for 16 h with 4 M methanesulfonic acid. Analytes were derivatized with methyl chloroformate and extracted into chloroform for GC/MS analysis. In addition to use of commercially available 13C-enriched Met and SeMet spikes for species specific ID analysis, a 74Se-enriched SeMet spike was also available for comparison of results. In selective ion monitoring mode, the intensities of ions at m/z 221, 222, 269, 270, and 263 were used to calculate the 221/222, 269/270, and 269/263 ion ratios for quantification of Met and SeMet. Concentrations of 5959 +/- 33 and 3404 +/- 12 microg g(-1) (one standard deviation, n = 6) with relative standard deviations of 0.55 and 0.36% for Met and SeMet, respectively, were obtained using 13C-enriched spikes. A concentration of 3417 +/- 8 microg g(-1) (one standard deviation, n = 6) was obtained using the 74Se-enriched SeMet spike. The concentration of SeMet measured in the yeast is equivalent to 66.43 +/- 0.24% of total Se and 30.31 +/- 0.11% of total Met is in the ...Continue Reading

References

Feb 1, 1993·The American Journal of Clinical Nutrition·B CorvilainJ E Dumont
May 1, 1997·The Journal of Nutrition·O A Levander
Jan 1, 1997·Nutrition and Cancer·M W RussoR S Sandler
Nov 26, 1998·American Journal of Epidemiology·P KnektA Aromaa
Jun 9, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·S W MyungK R Kim
Dec 5, 2002·Journal of Chromatography. a·H ChassaigneA R Rodriguez
Jan 10, 2003·Analytical and Bioanalytical Chemistry·Katarzyna WrobelJoseph A Caruso

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Citations

Jan 22, 2005·Analytical and Bioanalytical Chemistry·Aleksandra PołatajkoJoanna Szpunar
Apr 21, 2005·Analytical and Bioanalytical Chemistry·Heidi Goenaga InfanteTim Catterick
Nov 24, 2006·Analytical and Bioanalytical Chemistry·Wayne R Wolf, Robert J Goldschmidt
Dec 23, 2011·FEMS Yeast Research·Il-Kwon KimJens Nielsen
Nov 8, 2005·Mass Spectrometry Reviews·Ryszard LobińskiJoanna Szpunar
Mar 1, 2011·Analytical Methods : Advancing Methods and Applications·Michael StibollerC Oliver Kappe
Nov 28, 2008·Journal of Agricultural and Food Chemistry·Laurent Ouerdane, Zoltán Mester
Mar 23, 2006·Journal of Chromatography. a·C B'Hymer, J A Caruso
Feb 3, 2009·Analytica Chimica Acta·Zoyne Pedrero, Yolanda Madrid

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