Determination of metoprolol enantiomers in human plasma and saliva samples utilizing microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry

Biomedical Chromatography : BMC
Hatem ElmongyMohamed Abdel-Rehim

Abstract

A sensitive, accurate and reliable bioanalytical method for the enantioselective determination of metoprolol in plasma and saliva samples utilizing liquid chromatography-electrospray ionization tandem mass spectrometry was developed and validated. Human plasma and saliva samples were pretreated by microextraction by packed sorbent (MEPS) prior to analysis. A new MEPS syringe form with two inputs was used. Metoprolol enantiomers and internal standard pentycaine (IS) were eluted from MEPS sorbent using isopropanol after removal of matrix interferences using aliquots of 5% methanol in water. Complete separation of metoprolol enantiomers was achieved on a Cellulose-SB column (150 × 4.6 mm, 5 μm) using isocratic elution with mobile phase 0.1% ammonium hydroxide in hexane-isopropanol (80:20, v/v) with a flow rate of 0.8 mL/min. A post-column solvent-assisted ionization was applied to enhance metoprolol ionization signal in positive mode monitoring (+ES) using 0.5% formic acid in isopropanol at a flow rate of 0.2 mL/min. The total chromatographic run time was 10 min for each injection. The detection of metoprolol in plasma and saliva samples was performed using triple quadrupole tandem mass spectrometer in +ES under the following mass...Continue Reading

References

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Citations

Sep 13, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·T RosadoE Gallardo
Sep 14, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Olga FilippouVictoria Samanidou
Oct 11, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mohammad Mahdi MoeinMohamed Abdel-Rehim
Nov 9, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mariana Roberto Gama, Carla Beatriz Grespan Bottoli
Nov 12, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Liu YangMohamed Abdel-Rehim
Dec 14, 2017·Biomedical Chromatography : BMC·Michael G Bartlett
Dec 13, 2018·Bioanalysis·Shahram SeidiReza Alizadeh
Apr 18, 2021·Analytica Chimica Acta·Francisco Pena-PereiraElefteria Psillakis
Mar 5, 2021·Biomedical Chromatography : BMC·Seyed Mosayeb DaryanavardMohamed Abdel-Rehim

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