Determination of metronidazole in vaginal tissue by high-performance liquid chromatography using solid-phase extraction

Journal of Chromatography. B, Biomedical Applications
T G Venkateshwaran, J T Stewart

Abstract

A sensitive high-performance liquid chromatographic (HPLC) method for the determination of metronidazole in vaginal tissue is reported. The method uses a Zorbax SB phenyl column with a 0.01 M aqueous monobasic potassium phosphate buffer (pH 4.0)-absolute methanol (85:15, v/v) as mobile phase at a flow-rate of 1.0 ml/min and detection at 313 nm. Tinidazole was used as the internal standard. The method employed homogenization of tissue followed by solid-phase extraction. The quantitation was achieved within 30 min with sensitivity in the ng/g range. Metronidazole was linear in the 100-2000 ng/g range. The accuracy and precision were in the 1-4% range for the drug and the limit of detection was approximately 100 ng/g based on a signal-to-noise ratio of 3 and a 1009-microliters injection.

Citations

Mar 17, 2010·Analytical and Bioanalytical Chemistry·Maysa Faisal SuyaghJames C McElnay
Aug 28, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Han-Wen SunLian-Feng Ai
Jun 5, 2003·Veterinary Surgery : VS·Jose R RamosThomas J Inzana
Mar 31, 2005·Journal of Pharmaceutical and Biomedical Analysis·Ticiano Gomes do NascimentoRui Oliveira Macêdo
Jun 5, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yongqing WangHaibo Zhang
Aug 9, 2003·The Journal of Pharmacy and Pharmacology·Sanaa A El-Gizawy, Nagwa I Aglan
Oct 6, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·J D WibawaD A Barrett

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