Determination of Microbial Maintenance in Acetogenesis and Methanogenesis by Experimental and Modeling Techniques

Frontiers in Microbiology
Fabian BonkFlorian Centler

Abstract

For biogas-producing continuous stirred tank reactors, an increase in dilution rate increases the methane production rate as long as substrate input can be converted fully. However, higher dilution rates necessitate higher specific microbial growth rates, which are assumed to have a strong impact on the apparent microbial biomass yield due to cellular maintenance. To test this, we operated two reactors at 37°C in parallel at dilution rates of 0.18 and 0.07 days-1 (hydraulic retention times of 5.5 and 14 days, doubling times of 3.9 and 9.9 days in steady state) with identical inoculum and a mixture of volatile fatty acids as sole carbon sources. We evaluated the performance of the Anaerobic Digestion Model No. 1 (ADM1), a thermodynamic black box approach (TBA), and dynamic flux balance analysis (dFBA), to describe the experimental observations. All models overestimated the impact of dilution rate on the apparent microbial biomass yield when using default parameter values. Based on our analysis, a maintenance coefficient value below 0.2 kJ per carbon mole of microbial biomass per hour should be used for the TBA, corresponding to 0.12 mmol ATP per gram dry weight per hour for dFBA, which strongly deviates from the value of 9.8 kJ ...Continue Reading

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Citations

Jun 4, 2019·Frontiers in Microbiology·Sören WeinrichFlorian Centler
Jan 16, 2021·Microorganisms·Alexander Tøsdal TveitMette Marianne Svenning

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Datasets Mentioned

BETA
PRJEB22603
2

Methods Mentioned

BETA
amplicon sequencing
electrophoresis
PCR

Software Mentioned

QIIME
BBMerge
ADM1
jvenn
Matlab
dFBA
usearch
CellNetAnalyzer

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