Determination of microcystins in natural blooms and cyanobacterial strain cultures by matrix solid-phase dispersion and liquid chromatography-mass spectrometry

Analytical and Bioanalytical Chemistry
Ana María CameánY Picó

Abstract

An analytical procedure based on matrix solid-phase dispersion (MSPD) and liquid chromatography-mass spectrometry (LC-MS) was developed for determining three microcystins (MCs) in natural water blooms and cyanobacteria strain cultures. The procedure involves sample homogenization with C(18), washed with dichloromethane to eliminate interfering compounds, and elution with acidic methanol. Results were compared to those achieved by using an organic solvent standard method. Mean recoveries of MCs with MSPD were 85-92% with intra-day relative standard deviation (RSDs) of 9-19%, whereas organic solvent extraction resulted in recovery rates of 92-105% with intra-day RSDs ranging from 8 to 18%. Limits of quantification (LOQs) were 1 microg g(-1) dry weight for the MCs either by MSPD or organic solvent extraction. The two analytical methods tested were specific and sensitive to the extraction of MCs and were applied to the detection of MCs in water blooms and culture strains. The concentration of MCs varied from 7 to 3,330 microg g(-1) of lyophilized cells with MC-LR always showing the highest concentration. MCs levels were higher in culture strains than in water blooms, except for MC-LR, whose concentration in blooms was slightly supe...Continue Reading

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Citations

Feb 8, 2008·Analytical and Bioanalytical Chemistry·M García-LópezI Rodríguez
Jul 13, 2005·Journal of Chromatography. a·Isabel María MorenoAna María Cameán
Jul 20, 2013·Environmental Science & Technology·Ankita SrivastavaRavi Kumar Asthana
Mar 5, 2016·Archives of Environmental Contamination and Toxicology·Kennedy EguzozieEmmanuella C Nnabuo-Eguzozie
Jul 22, 2005·Journal of Mass Spectrometry : JMS
Nov 17, 2006·Journal of Biochemical and Biophysical Methods·Steven A Barker
May 31, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Remedios Guzmán-GuillénAna M Cameán
Feb 20, 2010·Journal of Separation Science·Marek Trojanowicz
Jan 12, 2011·Analytical and Bioanalytical Chemistry·Tomoharu SanoKunimitsu Kaya
Feb 3, 2005·Rapid Communications in Mass Spectrometry : RCM·Cariton KubwaboFrank M Benoit
Jul 27, 2005·Phytochemical Analysis : PCA
Feb 15, 2020·Toxicon : Official Journal of the International Society on Toxinology·Ning LuHongtao Lei
Jul 1, 2008·Toxicon : Official Journal of the International Society on Toxinology·L AtencioA M Cameán
Jun 15, 2006·Analytical Chemistry·Susan D Richardson

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