Determination of Minimal Hemoglobin Level Necessary for Normothermic Porcine Ex Situ Liver Perfusion

Transplantation
M BralA M James Shapiro

Abstract

In current studies of ex situ liver perfusion there exists considerable variability in perfusate composition, including the type of oxygen carrier. Herein, we aim to clarify the minimal hemoglobin level necessary during normothermic porcine ex situ liver perfusion. Livers procured from 35 to 45 kg domestic pigs were connected to our experimental ex situ circuit (n = 10). In the treatment group, perfusate was sequentially diluted hourly to predetermined hemoglobin levels. At the end of each hemoglobin dilution, perfusate samples were analyzed for liver transaminases, lactate dehydrogenase (LD), total bilirubin, and lactate levels. Liver oxygen consumption was measured. In the control group, livers were perfused continually for a duration of 24 hours at target hemoglobin levels of 30 and 20 g/L. Rising liver transaminases, significantly higher lactate (P < 0.001), and LD levels (P < 0.001) were noted at lower perfusate hemoglobin levels in the treatment group. Liver oxygen utilization (P < 0.001) and hepatic artery oxygen delivery (P < 0.001) were significantly lower at lower hemoglobin levels, whereas liver vessel resistance remained relatively constant. Histology demonstrated increasing parenchymal damage at lower hemoglobin le...Continue Reading

References

May 1, 2002·Transplantation·Andrew J ButlerPeter J Friend
Dec 20, 2003·Annals of Surgery·Peter L AbtJames F Markmann
Jun 30, 2009·Annals of Surgery·Jens BrockmannPeter J Friend
Dec 5, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J V GuarreraJ C Emond
Aug 25, 2011·Annals of Surgery·Constantino FondevilaJuan C García-Valdecasas
Nov 22, 2011·The Journal of Surgical Research·Hongzhi XuMartin Hertl
Sep 19, 2012·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Diethard MonbaliuJacques Pirenne
Mar 8, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·S op den DriesR J Porte
May 15, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M U BoehnertM Selzner
May 9, 2014·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Qiang LiuCristiano Quintini
Dec 3, 2014·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Christopher W WhiteDarren H Freed
Jan 24, 2015·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·P FontesJ W Marsh
Sep 24, 2015·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·C J E WatsonA J Butler
Nov 15, 2015·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Thamara PereraP Muiesan
Dec 15, 2015·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Q LiuC Quintini
Jan 12, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·R RavikumarP J Friend
Oct 28, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·H MergentalD F Mirza
Oct 27, 2016·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Markus SelznerDavid R Grant
Oct 22, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M BralA M J Shapiro
Nov 4, 2016·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Thomas VogelPeter J Friend
Jun 24, 2017·Transplantation·Paulo A Fontes
Dec 28, 2017·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Alix P M MattonRobert J Porte
Jan 9, 2018·Transplantation·Alvaro Rojas-Pena, Robert H Bartlett
Jan 13, 2018·Transplantation·Ahmed Nassar, Cristiano Quintini
Apr 20, 2018·Nature·David NasrallaUNKNOWN Consortium for Organ Preservation in Europe

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Citations

Aug 2, 2018·Expert Review of Gastroenterology & Hepatology·Kumar JayantA M James Shapiro
Apr 25, 2019·PloS One·Mariusz BralA M James Shapiro
Jan 1, 2021·International Journal of Molecular Sciences·Silke B BodewesRobert J Porte

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