Determination of mitoxantrone in rat plasma by liquid chromatography-tandem mass spectrometry method: Application to a pharmacokinetic study

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Peng ZhangZhonggui He

Abstract

A rapid, sensitive and specific high performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method has been developed for quantification of mitoxantrone in rat plasma. The analyte and palmatine (internal standard) were extracted from plasma samples with diethyl ether-dichloromethane (3:2, v/v) and separated on a C(18) column. The chromatographic separation was achieved within 2.5min using methanol-10mM ammonium acetate containing 0.1% acetic acid as the mobile phase at a flow rate of 0.2mL/min. The method was linear over the range of 0.5-500ng/mL. The lower limit of quantification (LLOQ) was 0.5ng/mL. Finally, the method was successfully applied to a pharmacokinetic study of mitoxantrone in rats following intravenous administration.

References

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Citations

Jun 28, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Peng ZhangZhonggui He
Aug 19, 2015·Mass Spectrometry Reviews·Sara CrottiMarco Agostini
May 20, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Lília R Cordeiro PedrosaGerben A Koning
Jun 1, 2017·The Analyst·Nicolas GuichardSandrine Fleury-Souverain
Jan 1, 2019·Critical Reviews in Analytical Chemistry·Sevinc KurbanogluSibel A Ozkan

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