Determination of mycophenolic acid plasma levels in renal transplant recipients co-administered sirolimus: comparison of an enzyme multiplied immunoassay technique (EMIT) and liquid chromatography-tandem mass spectrometry

Therapeutic Drug Monitoring
Aurélie PrémaudPierre Marquet

Abstract

This study was designed to compare the enzyme multiplied immunoassay technique (EMIT) and a specific ;liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of mycophenolic acid (MPA) concentrations in plasma samples collected from renal transplant patients receiving mycophenolate mofetil in association with sirolimus and corticosteroids. Ten-point blood concentration-time profiles, between pre-dose (C0) and 9-hour post-dose, were obtained on day 7, day 14, months 1, 2, and 3 posttransplantation in 8 patients. MPA plasma concentrations were measured simultaneously with both EMIT and LC-MS/MS. Higher concentrations were obtained with EMIT. The mean (+/-SD) absolute difference was +0.84 +/- 1.93 mg/L, which corresponds to a significant overestimation of 18.7 +/- 2 6.8% with EMIT, with variations depending on the time elapsed since transplantation. An EMIT overestimation of 16.7 +/- 22.5% also was obtained for the MPA areas under the curve calculated using the trapezoidal rule.

Citations

May 24, 2007·Analytical and Bioanalytical Chemistry·Franck Saint-MarcouxPierre Marquet
Feb 20, 2013·Journal of Molecular Modeling·Florent Di MeoPatrick Trouillas
Oct 16, 2007·Drug Discovery Today·Maggie A Z Hupcey, Sean Ekins
May 8, 2007·Clinical Biochemistry·Fawzy A Elbarbry, Ahmed S Shoker
Mar 17, 2016·Scandinavian Journal of Clinical and Laboratory Investigation·Ji Yeon HamJang-Hee Cho
Sep 19, 2006·Journal of Pharmaceutical and Biomedical Analysis·Fawzy A Elbarbry, Ahmed Shoker
Jan 3, 2007·Journal of Mass Spectrometry : JMS
Aug 21, 2007·Clinical Journal of the American Society of Nephrology : CJASN·Hyunyoung Jeong, Bruce Kaplan
Jan 9, 2010·Clinical Journal of the American Society of Nephrology : CJASN·Dirk R J KuypersUNKNOWN Transplantation Society (TTS) Consensus Group on TDM of MPA
Jan 6, 2019·European Journal of Clinical Pharmacology·Pâmela C Lukasewicz FerreiraPedro Eduardo Fröehlich
Jul 15, 2021·Analytical Chemistry·Álvaro Luque-UríaMaría C Moreno-Bondi
Mar 27, 2018·Analytical Chemistry·Bettina Glahn-MartínezMaría C Moreno-Bondi

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