Determination of N-nitrosodiethanolamine, NDELA in cosmetic ingredients and products by mixed mode solid phase extraction and UPLC-tandem mass spectrometry with porous graphitic carbon column through systemic sample pre-cleanup procedure

Talanta
Kyung-Mi JooKyung-Min Lim

Abstract

A rapid, sensitive, accurate and specific ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) method for the detection of N-nitrosodiethanolamine (NDELA), a highly toxic contaminant in cosmetic raw materials and products was developed and validated. Systematized sample preparation steps were developed according to product types. Various SPE cartridges and columns were examined to establish the condition of SPE and chromatographic separation for NDELA. Sample cleanup steps consisting of solvent and liquid-liquid extraction tailored to the various sample matrix types were established prior to mixed mode SPE (Bond Elut AccuCAT). Chromatographic separation was achieved within 7 min on a porous graphitic carbon (PGC) column using a gradient elution with the mobile phase of 1mM ammonium acetate containing 0.1% acetic acid and methanol. NDELA was monitored using an electrospray positive ionization mass spectrometry in the multiple reaction monitoring (MRM) mode (m/z 134.9>103.7(quantifier) and 73.7(qualifier ion)) with d8-NDELA (m/z 143.1>111.0) as internal standard. The standard curves were linear over the concentration range of 1-100 ng/mL with a correlation coefficient higher than 0.99. The limi...Continue Reading

References

Jan 1, 1984·Journal of Cancer Research and Clinical Oncology·B Spiegelhalder, R Preussmann
Nov 25, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Cyrille A M KrulRobert Havenaar
Dec 1, 2011·Toxicological Research·Du Yeon BangByung-Mu Lee
Apr 2, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sylwia MagieraJacek Baranowski

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Citations

Jun 24, 2016·Journal of Separation Science·Zhixiong Zhong, Gongke Li
Feb 24, 2018·Journal of Toxicology and Environmental Health. Part a·Duck Soo LimByung-Mu Lee
Apr 26, 2018·Journal of Toxicology and Environmental Health. Part a·Duck Soo LimByung-Mu Lee
Aug 11, 2020·Critical Reviews in Food Science and Nutrition·Yu BianXue-Song Feng
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria CeleiroMarta Lores

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