PMID: 8585330Dec 1, 1995Paper

Determination of naturally occurring sex steroid hormones (androgens and progestagens) in beef

Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung
M HartwigH Steinhart

Abstract

The content of 10 naturally occurring steroidal sex hormones (androgens and progestogens), their biosynthetic precursors and metabolites was determined in 62 samples of beef (bulls, steers, heifers). After enzymatic hydrolysis of their conjugates, the steroids were extracted from the tissue by liquid-liquid extraction and purified by solid phase extraction. The identification and quantification was carried out by GC-MS of the trimethylsilyl ethers. The progestogens progesterone and pregnenolone were quantitatively dominant (43.7 and 6.5 micrograms/kg respectively). The highest steroid concentrations were determined in female cattle.

Citations

May 28, 2015·Analytical and Bioanalytical Chemistry·Leonardo Di DonnaFabio Mazzotti
Jan 1, 2002·Meat Science·R Zeleny, H Schimmel
Mar 23, 2001·Journal of Agricultural and Food Chemistry·A PrombergerA Jungbauer

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