Determination of nine active components in Radix Hedysari and Radix Astragali using capillary HPLC with diode array detection and MS detection

Journal of Separation Science
Jing ZhaoWei Wang

Abstract

A capillary HPLC (cHPLC) coupled with diode array detection (DAD) and MS method was developed for the simultaneously qualitative and quantitative determination of nine components, namely vanillic acid, calycosin-7-O-beta-D-glucoside, (6alphaR,11alphaR)-9,10-dimethoxypterocarpan-3-O-beta-D-glucoside, ononin, calycosin, (3R)-2'-hydroxy-3',4'-dimethoxyisoflavan-7-O-beta-D-glucoside, isoliquiritigenin, formononetin, (3R)-8,2'-dihydroxy-7,4'-dimethoxyisoflavan, in Radix Hedysari (Hongqi) and Radix Astragali (Huangqi). Simultaneous separation of these nine compounds was achieved on a Zorbax C18 microcolumn (5 microm, 150 x 0.3 mm). The mobile phase consisted of (A) 0.3% aqueous formic acid and (B) ACN with a gradient elution. The identification of nine compounds in both Hongqi and Huangqi was confirmed by TOF-MS. All calibration curves showed good linearity (R(2) >0.998) within test ranges. This method showed good repeatability for the quantification of these nine components in Hongqi and Huangqi with intra- and inter-day variations of less than 1.89 and 3.13%, respectively. The validated method was successfully applied to quantify nine investigated components in eighteen samples of Hongqi and Huangqi. Hierarchical cluster analysis o...Continue Reading

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Citations

Mar 13, 2010·Journal of Biomedicine & Biotechnology·Jing LiuHubiao Chen
Dec 5, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Guan-Cheng HuangChing-Chiung Wang
Jun 12, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Wendy Li ZhangKarl Wah-Keung Tsim
Aug 12, 2014·Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan·Jing ZhouXu Zhang
Jan 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuan LiJin-Ao Duan
Oct 16, 2010·Immunopharmacology and Immunotoxicology·Na-Hyung KimHyun-Ja Jeong
Sep 6, 2008·Phytochemical Analysis : PCA

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