Determination of nine pyrethroid insecticides by high-performance liquid chromatography with post-column photoderivatization and detection based on acetonitrile chemiluminescence

Journal of Chromatography. a
M Martínez GaleraR Santiago Valverde

Abstract

An HPLC method was developed to determine pyrethroids, including fenpropathrin, beta-cyfluthrin, lambda-cyhalothrin, deltamethrin, fenvalerate, permethrin, acrinathrin, tau-fluvalinate, and bifenthrin, by coupling HPLC, post-column irradiation with UV light and chemiluminescence detection of the resulting photoproducts. It is based on the observation that photolyzed pyrethroids take part in a chemiluminescent reaction in presence of K3Fe(CN)6 and NaOH, whose signal increases with the percentage of acetonitrile in the reaction medium. As the yield of the photoderivatization process and the chemiluminescent signals depend on the percentage of acetonitrile, the chromatographic column (a Gemini C18, Phenomenex 150 mm x 4.6 mm, 5 microm particle size) was chosen with the aim of using high percentages of this organic solvent in the mobile phase. Previous studies showed that the rate of the chemiluminescent reaction was very fast. Therefore, a modification was carried out in the detector in order to mix the analytes and reactives as near as possible to the measure cell. The optimised method was validated with respect to linearity, precision, limits of detection and quantification accuracy. Under the optimised conditions, linear workin...Continue Reading

References

Sep 25, 2001·Analytical Chemistry·L Lahuerta ZamoraJ Martínez Calatayud

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Citations

Oct 19, 2010·Analytica Chimica Acta·Francisco J LaraJean-Jacques Aaron
Jun 24, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yuan LiangHuwei Liu
Feb 23, 2007·Journal of Separation Science·Lidia María Ravelo-PérezMiguel Angel Rodríguez-Delgado
Mar 23, 2011·Drug Testing and Analysis·Jacqui L AdcockPaul S Francis
Jan 8, 2013·Environmental Pollution·Heather M ColemanStuart J Khan
Apr 14, 2012·Journal of Chromatographic Science·Shuliang LiuHuaiyu Yuan
Apr 16, 2016·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·M Catalá-IcardoS Torres-Cartas
Oct 19, 2010·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Z M GrahovacA N Pavlović

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