Determination of nucleic acids based on shifting the association equilibrium between tetrasulfonated aluminium phthalocyanine and acridine orange

The Analyst
D H LiJ G Xu

Abstract

Based on the ability of nucleic acids to shift the association equilibrium of the ion-association complex of Acridine Orange and tetrasulfonated aluminium phthalocyanine, thus leading to an increase in the phthalocyanine fluorescence, a method is suggested for the fluorimetric determination of nucleic acids. Investigations were carried out on the spectral characteristics, order of addition of reagents, selection of the buffer system, effect of pH, influence of reaction time, effect of salt, the usage of reagents, interference of foreign substances and the effect of different acridine derivatives. Under the optimum conditions, the calibration graphs for the determination of calf thymus DNA (CT DNA), salmon DNA (SM DNA) and yeast RNA were linear over the ranges 0.04-1.2, 0.04-1.2 and 0.1-1.2 micrograms cm-1, respectively. The detection limits for CT DNA, SM DNA and RNA were 17, 24 and 98 micrograms cm-3, respectively. The relative standard deviation (n = 6) was within 4.6% for the detection of samples. The method was applied to the determination of Staphylococcus aureus DNA and the result was in agreement with that achieved by a UV method.

Citations

May 23, 2012·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Junfen LiMartin M F Choi
Mar 24, 2009·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Junfen LiChuan Dong
Sep 27, 2008·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·J F Li, C Dong
Jan 15, 2005·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Changqing ZhuJingou Xu
Apr 29, 2006·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Xia WuJinbo Wu
Feb 15, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Guang-Xin DuDong-Hui Li

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