PMID: 15228116Jul 2, 2004Paper

Determination of nucleic acids by near-infrared fluorescence quenching of hydrophobic thiacyanine dye in the presence of Triton X-100

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Chang-Qing ZhuJingou Xu

Abstract

A near-infrared (near-IR) fluorescence quenching method was developed for the determination of nucleic acids in aqueous solution by using a cationic heptamethylene thiacyanine as a probe. The near-IR cationic cyanine showed maximum excitation and emission wavelengths at 800 and 825 nm, respectively, in the presence of Triton X-100; the fluorescence of the cyanine could be greatly quenched by DNA. The calibration graphs were linear over the range of 10-400 ng/mL for CT (calf thymus) DNA and over the range 5-400 ng/mL for FS (fish sperm) DNA under optimal conditions. The corresponding detection limits were 5.2 ng/mL for CT DNA and 2.5 ng/mL for FS DNA. The relative standard deviation (n = 8) was 3.1% for 75 ng/mL CT DNA and 2.2% for 75 ng/mL FS DNA, respectively. Preliminary research showed that the fluorescence quenching might be ascribed to the formation of dye aggregate facilitated by DNA.

References

Jun 1, 1989·Analytical Biochemistry·G A DaxheletC G Cocito
Jun 1, 1985·Analytical Biochemistry·M Legros, A Kepes
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
May 4, 2000·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy· Ogul'chansky TYuS M Yarmoluk
Feb 28, 2001·Fresenius' Journal of Analytical Chemistry·H ZhengJ G Xu
Jun 5, 2003·Analytical Biochemistry·Hong ZhengChang Qing Zhu

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Citations

Jun 15, 2006·Analytical Chemistry·Kristin A FletcherIsiah M Warner
May 9, 2009·Luminescence : the Journal of Biological and Chemical Luminescence·Yuan LiuJinghe Yang

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