Determination of ofloxacin and moxifloxacin and their penetration in human aqueous and vitreous humor by using high-performance liquid chromatography fluorescence detection

Analytical Biochemistry
Kwok Ping ChanChi Pui Pang

Abstract

Information on comparing the penetration of ofloxacin and moxifloxacin in the human eye is unavailable, although these two antibiotics are commonly used in ophthalmic surgery. There is a need for a rapid, reliable, and sensitive methodology for their determination in ocular fluids. We developed a robust HPLC procedure with fluorescence detection for simultaneous analysis of ofloxacin and moxifloxacin in human and rabbit aqueous and vitreous samples. The linearity of the method ranged from 10 ng/ml to 100 microg/ml with r(2) > 0.996. Most inter- and intrabatch imprecision was about 5% (range 1.6-7.6%), recoveries between 95 and 104%, and accuracies between 93 and 104% at 0.1 and 1 microg/ml. The detection limits of both compounds were 10 ng/ml (0.028 nmol/ml for ofloxacin and 0.023 nmol/ml for moxifloxacin). No sample treatment was necessary for aqueous humor and only acetonitrile precipitation was required for vitreous humor. The chromatographic time was short, 22 min. We applied this method to study penetrations of ofloxacin and moxifloxacin in aqueous and vitreous humors of human and rabbits. There was no significant difference of penetration between the two antibiotics into aqueous and vitreous but ofloxacin was found at sig...Continue Reading

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Citations

Sep 26, 2012·Bulletin of Environmental Contamination and Toxicology·Te-San ChenJia-Long Chen
Jul 17, 2015·Biomedical Chromatography : BMC·Jing NieHong-Ping Xu
Mar 14, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Fahim Ullah KhanFarhad Ullah
May 1, 2009·Bioanalysis·Marilyn J Schneider
Jan 18, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Safwan Ashour, Roula Bayram
Sep 6, 2019·Mikrochimica Acta·Haoyang YiShanshan Zhou
Mar 28, 2008·Journal of Medicinal Chemistry·Anton SimeonovJames Inglese

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